DDX3 Antibody (2253C5a): sc-81247

DDX3 Antibody (2253C5a) is a mouse monoclonal IgG1 kappa light chain antibody that detects the DDX3X protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-DDX3 antibody (2253C5a) is available as the non-conjugated form. The DDX3X protein, a member of the DEAD box protein family, plays a crucial role in RNA metabolism and is characterized by the conserved Asp-Glu-Ala-Asp motif, which is essential for its function as a putative RNA helicase. DDX3X is predominantly localized to nuclear speckles, where DDX3X is involved in various cellular processes, including RNA splicing, transcription, translation initiation, mRNA transport, and cell cycle regulation. DDX3X′s importance extends to its involvement in viral infections, such as HIV-1 and hepatitis C, highlighting DDX3X′s potential as a therapeutic target. Additionally, DDX3X has two paralogs in humans, with DDX3Y being exclusively expressed in the testis and essential for normal spermatogenesis. The diverse functions and interactions of DDX3X underscore DDX3X′s significance in both normal cellular physiology and disease states, making DDX3 (2253C5a) antibody a valuable tool for researchers studying RNA dynamics and related pathways.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

DDX3 Antibody (2253C5a) References:

1. DDX3, a DEAD box RNA helicase with tumor growth-suppressive property and transcriptional regulation activity of the p21waf1/cip1 promoter, is a candidate tumor suppressor.  |  Chao, CH., et al. 2006. Cancer Res. 66: 6579-88. PMID: 16818630
2. Human DEAD-box ATPase DDX3 shows a relaxed nucleoside substrate specificity.  |  Franca, R., et al. 2007. Proteins. 67: 1128-37. PMID: 17357160
3. Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin B/cdc2 in vitro.  |  Sekiguchi, T., et al. 2007. Biochem Biophys Res Commun. 356: 668-73. PMID: 17379183
4. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the DDX3 RNA helicase domain.  |  Rodamilans, B. and Montoya, G. 2007. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63: 283-6. PMID: 17401195
5. Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMP.  |  Högbom, M., et al. 2007. J Mol Biol. 372: 150-9. PMID: 17631897
6. Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and Rev.  |  Nekhai, S. and Jeang, KT. 2006. Future Microbiol. 1: 417-26. PMID: 17661632
7. Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein.  |  Shih, JW., et al. 2008. Oncogene. 27: 700-14. PMID: 17667941
8. DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.  |  Ariumi, Y., et al. 2007. J Virol. 81: 13922-6. PMID: 17855521
9. The DDX3 subfamily of the DEAD box helicases: divergent roles as unveiled by studying different organisms and in vitro assays.  |  Rosner, A. and Rinkevich, B. 2007. Curr Med Chem. 14: 2517-25. PMID: 17979704
10. DDX3-mediated miR-34 expression inhibits autophagy and HBV replication in hepatic cells.  |  Mishra, AK., et al. 2023. J Viral Hepat. 30: 327-334. PMID: 36597176
11. RNA helicase DDX3 regulates RAD51 localization and DNA damage repair in Ewing sarcoma.  |  Randolph, ME., et al. 2024. iScience. 27: 108925. PMID: 38323009
12. Arginine Methylation of DDX3 by PRMT1 Mediates Mitochondrial Homeostasis to Promote Breast Cancer Metastasis.  |  Hsu, WJ., et al. 2024. Cancer Res. 84: 3023-3043. PMID: 39042374

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX3 Antibody (2253C5a) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX3 Antibody (2253C5a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX3 Antibody (2253C5a) Specifications

Alternate Product Name: DDX3

Product Clone Name: 2253C5a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: DDX3

Epitope Species: human

Product Reactivity/Detection: DDX3X

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for DDX3 Antibody (2253C5a): N/A