Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DAPK Double Nickase Plasmid (h): sc-402702-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAPK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DAPK Double Nickase Plasmid (h) and DAPK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DAPK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAPK Antibody (17): sc-136286
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAPK Double Nickase Plasmid (h) | sc-402702-NIC | 20 µg | $410.00 | |||
DAPK Double Nickase Plasmid (h2) | sc-402702-NIC-2 | 20 µg | $410.00 |
Death-associated protein kinase 1 (DAPK1) encodes DAPK, a Ca2+/calmodulin-regulated serine/threonine kinase that integrates apoptotic and autophagic signaling with cytoskeletal dynamics. DAPK modulates cell death pathways through phosphorylation of multiple substrates, influencing stress responses, anoikis, and mitochondrial-dependent apoptosis, and it interfaces with MAPK and inflammatory signaling networks. Altered DAPK1 activity or expression has been associated with dysregulated cell survival and migration programs, linking it to disease-relevant processes such as tumor suppression, neurodegeneration-related stress signaling, and immune-mediated tissue injury. As a nodal regulator of programmed cell death, DAPK1 is frequently studied to dissect pathway cross-talk that shapes cellular fate decisions under genotoxic, oxidative, or cytokine stress.
DAPK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DAPK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DAPK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DAPK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DAPK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.