DABP CRISPR/Cas9 KO Plasmid (h): sc-405222
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DABP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DABP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DABP Antibody (H-6): sc-390146
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DABP CRISPR/Cas9 KO Plasmid (h) | sc-405222 | 20 µg | $397.00 |
Overview
Human DBP encodes the vitamin D–binding protein (DABP, also known as group-specific component), a highly abundant plasma glycoprotein that transports vitamin D metabolites and contributes to regulation of their bioavailability. Beyond carrier function, DABP participates in actin scavenging during tissue injury and interfaces with innate immune processes through macrophage activation–related activities. Variation in DBP has been investigated in contexts of altered vitamin D homeostasis and inflammatory phenotypes, supporting its use in studies of endocrine-immune crosstalk. DBP expression and DABP-mediated ligand binding are therefore relevant to pathways governing vitamin D metabolism, barrier and tissue damage responses, and systemic inflammatory signaling.
DABP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DBP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DBP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DBP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DABP protein expression.
This CRISPR knockout system enables efficient generation of DBP-deficient cell models for investigation of DABP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DBP exon(s) critical for DABP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DBP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DABP CRISPR/Cas9 KO Plasmid (h) and DABP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DBP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DABP HDR Plasmid (h) and DABP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DBP homology arms to support homology-directed repair at defined DBP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.