D54 CRISPR/Cas9 KO Plasmid (m): sc-425948
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- D54 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the D54 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
D54 CRISPR/Cas9 KO Plasmid (m) | sc-425948 | 20 µg | $397.00 |
Overview
Tpd52l2 encodes D54, a member of the tumor protein D52 family implicated in regulation of vesicular trafficking, membrane dynamics, and secretory processes that influence cell growth and differentiation. D54 has been linked to pathways governing intracellular transport and cytoskeletal organization, supporting coordinated delivery of signaling components and membrane proteins. Altered expression of D52 family proteins is frequently observed in proliferative and metabolic contexts, making Tpd52l2 a useful node for studying dysregulated cell-cycle control and stress-adaptive remodeling. In mouse systems, Tpd52l2 provides a tractable target for dissecting how trafficking-associated proteins modulate signaling output and tissue homeostasis in disease-relevant models.
D54 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tpd52l2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tpd52l2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tpd52l2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish D54 protein expression.
This CRISPR knockout system enables efficient generation of Tpd52l2-deficient cell models for investigation of D54 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tpd52l2 exon(s) critical for D54 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tpd52l2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by D54 CRISPR/Cas9 KO Plasmid (m) and D54 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tpd52l2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by D54 HDR Plasmid (m) and D54 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tpd52l2 homology arms to support homology-directed repair at defined Tpd52l2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.