Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 19 CRISPR/Cas9 KO Plasmid (h): sc-400281
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 19 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cytokeratin 19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 19 Antibody (A-3): sc-376126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 19 CRISPR/Cas9 KO Plasmid (h) | sc-400281 | 20 µg | $397.00 |
Overview
KRT19 encodes cytokeratin 19 (CK19), a type I intermediate filament protein that partners with type II keratins to form the keratin cytoskeleton in epithelial cells. CK19 supports structural integrity and mechanotransduction, contributing to cell polarity, adhesion dynamics, and stress responses through interactions with desmosomal and hemidesmosomal complexes. Altered KRT19 expression is associated with epithelial differentiation states and remodeling programs such as wound repair and epithelial–mesenchymal transition, linking it to changes in proliferation, migration, and cellular plasticity. In cancer biology, CK19 is widely used as an epithelial lineage marker and is frequently studied in tumor progression and metastasis-related phenotypes.
Cytokeratin 19 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KRT19 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KRT19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KRT19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cytokeratin 19 protein expression.
This CRISPR knockout system enables efficient generation of KRT19-deficient cell models for investigation of Cytokeratin 19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KRT19 exon(s) critical for Cytokeratin 19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KRT19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cytokeratin 19 CRISPR/Cas9 KO Plasmid (h) and Cytokeratin 19 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KRT19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cytokeratin 19 HDR Plasmid (h) and Cytokeratin 19 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KRT19 homology arms to support homology-directed repair at defined KRT19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.