Cytokeratin 1 Anticuerpo (4D12B3) es un monoclonal de ratón IgG1 κ, ver las 13 publicaciones, proporcionado como 200 µg/ml
raised against purified truncated recombinant Cytokeratin 1 of human origin
Cytokeratin 1 Anticuerpo (4D12B3) es recomendado para detectar Cytokeratin 1 de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)
Cytokeratin 1 Anticuerpo (4D12B3) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cytokeratin 1 (4D12B3): sc-65999.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for Cytokeratin 1 Antibody (4D12B3) for WB and IHC(P) applications. These reagents are now offered in bundles with Cytokeratin 1 Antibody (4D12B3) (see ordering information below).
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Labware ships FedEx Ground free of charge to the contiguous US
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can Cytokeratin 1 (4D12B3): sc-65999 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Preguntado por: Cweed
Thank you for your question. Yes, Cytokeratin 1 (4D12B3): sc-65999 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 5 de
K Harmon de
Great, Versatile AntibodyThis antibody worked great for our lab on FFPE canine tissues using a variety of conditions. Retrieval worked equally well using both EDTA pH9 and citrate pH6 buffers, and signal was still quite strong at a 1:500 dilution, though somewhere between 1:250 and 1:500 would probably be optimal.
Fecha de publicación: 2021-02-10
Rated 1 de
Didn't work well for IHCThis antibody didn't work well on formalin fixed, paraffin-embedded mouse skin tissue.
Fecha de publicación: 2017-08-10
Rated 4 de
ParaffinParaffin-embedded IHC publication using human keratinocytes. -SCBT Publication Review
Fecha de publicación: 2015-03-18
Rated 4 de
WB publication using HaCaT cell line (keratinocytes)WB publication using HaCaT cell line (keratinocytes) -SCBT Publication Review
Fecha de publicación: 2015-03-13
Rated 4 de
Signal via immunoperoxidase staining of formalinSignal via immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing cytoplasmic staining of squamous epithelial cells. -SCBT QC
Fecha de publicación: 2015-02-22
Rated 4 de
Positive western blot analysis of truncatedPositive western blot analysis of truncated human recombinant Cytokeratin 1 protein. -SCBT QC
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