cyclin T1 Antibody (E-6): sc-271576

Cyclin T1 Antibody (E-6) is a mouse monoclonal IgG2b antibody that detects cyclin T1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cyclin T1 plays a crucial role in the regulation of transcription elongation by forming a complex with cyclin-dependent kinase 9 (Cdk9), which is essential for the phosphorylation of the C-terminal domain of RNA polymerase II. This phosphorylation is vital for the transition of RNA polymerase II from transcription initiation to elongation, thereby facilitating the production of mRNA. The interaction between cyclin T1 and the HIV-1 Tat protein is particularly significant, as cyclin T1 enhances Tat′s binding affinity to the viral TAR RNA, allowing for the recruitment of the cyclin T1/Cdk9 complex to RNA polymerase II. This mechanism underscores cyclin T1′s importance in both cellular transcription processes and viral pathogenesis, making cyclin T1 a key target for research in gene expression and HIV biology. Anti-cyclin T1 antibody (E-6) serves as an invaluable tool for studying these complex interactions and their implications in various biological contexts.

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cyclin T1 Antibody (E-6) References:

1. Cyclin T1 domains involved in complex formation with Tat and TAR RNA are critical for tat-activation.  |  Ivanov, D., et al. 1999. J Mol Biol. 288: 41-56. PMID: 10329125
2. Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome.  |  Kiernan, RE., et al. 2001. Mol Cell Biol. 21: 7956-70. PMID: 11689688
3. CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation.  |  Bellan, C., et al. 2004. J Pathol. 203: 946-52. PMID: 15258998
4. Acetylation of cyclin T1 regulates the equilibrium between active and inactive P-TEFb in cells.  |  Cho, S., et al. 2009. EMBO J. 28: 1407-17. PMID: 19387490
5. Cyclin T1/CDK9 interacts with influenza A virus polymerase and facilitates its association with cellular RNA polymerase II.  |  Zhang, J., et al. 2010. J Virol. 84: 12619-27. PMID: 20943989
6. Limited redundancy in genes regulated by Cyclin T2 and Cyclin T1.  |  Ramakrishnan, R., et al. 2011. BMC Res Notes. 4: 260. PMID: 21791050
7. Identification of novel inhibitors against Cyclin Dependent Kinase 9/Cyclin T1 complex as: Anti cancer agent.  |  Hussain, A., et al. 2017. Saudi J Biol Sci. 24: 1229-1242. PMID: 28855816
8. The CDK9-cyclin T1 complex mediates saturated fatty acid-induced vascular calcification by inducing expression of the transcription factor CHOP.  |  Shiozaki, Y., et al. 2018. J Biol Chem. 293: 17008-17020. PMID: 30209133
9. A conditional RNA Pol II mono-promoter drives HIV-inducible, CRISPR-mediated cyclin T1 suppression and HIV inhibition.  |  Chinnapaiyan, S., et al. 2023. Mol Ther Nucleic Acids. 32: 553-565. PMID: 37215150
10. Upregulation of cyclin T1/CDK9 complexes during T cell activation.  |  Garriga, J., et al. 1998. Oncogene. 17: 3093-102. PMID: 9872325

The preferred cyclin T1 antibody is cyclin T1 Antibody (E-3): sc-271348.