cyclin G1 Double Nickase Plasmid (h): sc-402045-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin G1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cyclin G1 Double Nickase Plasmid (h) and cyclin G1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCNG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin G1 Antibody (F-5): sc-8016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin G1 Double Nickase Plasmid (h) | sc-402045-NIC | 20 µg | $410.00 | |||
cyclin G1 Double Nickase Plasmid (h2) | sc-402045-NIC-2 | 20 µg | $410.00 |
CCNG1 encodes cyclin G1, an atypical cyclin transcriptionally regulated by p53 that coordinates cell-cycle control with cellular stress responses. Cyclin G1 participates in checkpoint regulation and modulation of protein phosphorylation networks, including interactions that influence MDM2–p53 feedback and phosphatase-associated signaling such as PP2A-linked complexes. Through these pathways, CCNG1 affects proliferation, genomic stability, and responses to DNA damage, making it relevant to studies of tumor biology, resistance to genotoxic stress, and aberrant cell-cycle regulation in human disease contexts. Altered CCNG1 expression or regulation has been observed across multiple cancer types, supporting its use as a mechanistic node for dissecting stress-adaptive signaling.
cyclin G1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCNG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCNG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCNG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCNG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.