CCNB1/cyclin B1 Antibody (V92.1): sc-53238

Cyclin B1 Antibody (V92.1) is a mouse monoclonal IgG1 kappa light chain antibody raised against hamster-origin cyclin B1, reacting with mouse, rat, hamster, and human species. Anti-cyclin B1 antibody (V92.1) is suitable for applications such as western blotting (WB) and immunoprecipitation (IP). Cyclin B1, also known as G2/mitotic-specific cyclin-B1 or CCNB1, is a regulatory protein essential for controlling the transition from the G2 phase to mitosis during the cell cycle. Cyclin B1 forms a complex with cyclin-dependent kinase 1 (CDK1), also known as CDC2, creating the maturation-promoting factor (MPF) that initiates mitosis by phosphorylating target substrates. Cyclin B1 interacts with several key proteins, including Cdc25C phosphatase, which activates the cyclin B1/CDK1 complex by dephosphorylating CDK1, and Wee1 kinase, which inhibits CDK1 through phosphorylation. Cyclin B1 associates with 14-3-3 proteins that regulate subcellular localization and stability. These interactions are crucial for the precise timing and regulation of mitosis, and dysregulation can lead to uncontrolled cell proliferation associated with cancer. Understanding cyclin B1 and protein interactions is important for research into cell cycle regulation and the development of therapeutic interventions targeting proliferative diseases. Cyclin B1 (V92.1) antibody enables researchers to investigate these processes in various biological contexts, aiding in the advancement of cell biology and oncology studies.

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CCNB1/cyclin B1 Antibody (V92.1) References:

1. Integrative analysis of cyclin protein levels identifies cyclin b1 as a classifier and predictor of outcomes in breast cancer.  |  Agarwal, R., et al. 2009. Clin Cancer Res. 15: 3654-62. PMID: 19470724
2. Determinants of human cyclin B1 association with mitotic chromosomes.  |  Pfaff, KL. and King, RW. 2013. PLoS One. 8: e59169. PMID: 23505570
3. Cyclin B1 overexpression induces cell death independent of mitotic arrest.  |  Eichhorn, JM., et al. 2014. PLoS One. 9: e113283. PMID: 25415322
4. Defective Cyclin B1 Induction in Trastuzumab-emtansine (T-DM1) Acquired Resistance in HER2-positive Breast Cancer.  |  Sabbaghi, M., et al. 2017. Clin Cancer Res. 23: 7006-7019. PMID: 28821558
5. Cold-inducible RNA-binding protein regulates cyclin B1 against spermatogenesis arrest caused by heat stress.  |  Liu, H., et al. 2022. Andrology. 10: 392-403. PMID: 34628721
6. Porphyromonas gingivalis lipopolysaccharide enhances the proliferation of human periodontal ligament cells via upregulation of cyclin D1, cyclin A and cyclin B1.  |  Lu, J., et al. 2022. Exp Ther Med. 23: 2. PMID: 34815754
7. Cell cycle-dependent binding between Cyclin B1 and Cdk1 revealed by time-resolved fluorescence correlation spectroscopy.  |  Barbiero, M., et al. 2022. Open Biol. 12: 220057. PMID: 35765818
8. Dissecting the roles of the Tuberin protein in the subcellular localization of the G2/M Cyclin, Cyclin B1.  |  Pillon, A., et al. 2022. PLoS One. 17: e0272741. PMID: 35947627
9. KCNN1 promotes proliferation and metastasis of breast cancer via ERLIN2-mediated stabilization and K63-dependent ubiquitination of Cyclin B1.  |  Xiao, B., et al. 2023. Carcinogenesis. 44: 809-823. PMID: 37831636
10. Hyperactivation of p53 contributes to mitotic catastrophe in podocytes through regulation of the Wee1/CDK1/cyclin B1 axis.  |  Feng, J., et al. 2024. Ren Fail. 46: 2365408. PMID: 38874119

The preferred CCNB1/cyclin B1 antibody is CCNB1/cyclin B1 Antibody (GNS1): sc-245.