cyclin B1 Anticuerpo (0.N.238) es un monoclonal de ratón IgG1 κ, ver las 14 publicaciones, proporcionado como 200 µg/ml
raised against a recombinant protein corresponding to human cyclin B1
recomendado para detectarcyclin B1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y KA
Ver cyclin B1 (GNS1): sc-245 para cyclin B1 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for cyclin B1 Antibody (0.N.238) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin B1 Antibody (0.N.238) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
cyclin B1 para análisis funcionales de las repuestas celulares a Cyclin
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In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
How much sample do you recommend loading on the gel for Western blot detection using cyclin B1 (0.N.238): sc-70898?
Preguntado por: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
Rated 4 de
Band at expected size with transfected lysateBand at expected size with transfected lysate and K-562 nuclear extract -SCBT QC
Fecha de publicación: 2015-01-30
Rated 5 de
Published data in IF application with U2OSPublished data in IF application with U2OS cell lines with and without the constitutive expression of Epstein-Barr virus (EBV) EBNA1 protein and polyomavirus large T antigen -SCBT Publication Review
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