cyclin B1 Anticuerpo (0.N.238) recomendado para detectarcyclin B1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y KA

cyclin B1 Anticuerpo (0.N.238): sc-70898

cyclin B1 Anticuerpo (0.N.238) clasificado 4.5 de 5 por 2.
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5
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4
1
3
0
2
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1
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Ficha técnica

    • cyclin B1 Anticuerpo (0.N.238) es un monoclonal de ratón IgG1 κ, ver las 14 publicaciones, proporcionado como 200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • recomendado para detectarcyclin B1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y KA
    • Ver cyclin B1 (GNS1): sc-245 para cyclin B1 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for cyclin B1 Antibody (0.N.238) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin B1 Antibody (0.N.238) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using cyclin B1 (0.N.238): sc-70898?

Preguntado por: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-03-01
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Rated 4 de 5 por de Band at expected size with transfected lysate Band at expected size with transfected lysate and K-562 nuclear extract -SCBT QC
Fecha de publicación: 2015-01-30
Rated 5 de 5 por de Published data in IF application with U2OS Published data in IF application with U2OS cell lines with and without the constitutive expression of Epstein-Barr virus (EBV) EBNA1 protein and polyomavirus large T antigen -SCBT Publication Review
Fecha de publicación: 2013-01-22
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