CTDSPL2 CRISPR/Cas9 KO Plasmid (m): sc-435953
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTDSPL2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CTDSPL2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTDSPL2 CRISPR/Cas9 KO Plasmid (m) | sc-435953 | 20 µg | $397.00 |
Overview
Ctdspl2 encodes CTDSPL2, a small C-terminal domain phosphatase–like protein implicated in regulating phosphorylation-dependent signaling and transcriptional programs. Members of this phosphatase family are associated with control of RNA polymerase II CTD dephosphorylation dynamics and broader phospho-regulatory circuits that influence cell-cycle progression, differentiation, and maintenance of cellular identity. In mouse systems, CTDSPL2 is used as a mechanistic entry point to study how phosphatase-mediated switches integrate with transcriptional control and chromatin-associated processes. Altered regulation of CTD phosphatases and related signaling nodes is relevant to models of aberrant proliferation and lineage dysregulation, supporting investigation in oncology- and development-oriented research contexts.
CTDSPL2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ctdspl2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ctdspl2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ctdspl2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CTDSPL2 protein expression.
This CRISPR knockout system enables efficient generation of Ctdspl2-deficient cell models for investigation of CTDSPL2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ctdspl2 exon(s) critical for CTDSPL2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ctdspl2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CTDSPL2 CRISPR/Cas9 KO Plasmid (m) and CTDSPL2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ctdspl2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CTDSPL2 HDR Plasmid (m) and CTDSPL2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ctdspl2 homology arms to support homology-directed repair at defined Ctdspl2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.