Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CtBP1 Double Nickase Plasmid (h): sc-400667-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CtBP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CtBP1 Double Nickase Plasmid (h) and CtBP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CtBP1 Antibody (G-6): sc-398945
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CtBP1 Double Nickase Plasmid (h) | sc-400667-NIC | 20 µg | $410.00 | |||
CtBP1 Double Nickase Plasmid (h2) | sc-400667-NIC-2 | 20 µg | $410.00 |
CTBP1 encodes the transcriptional corepressor CtBP1, a conserved regulator that links DNA-binding repressors to chromatin-modifying complexes to shape gene expression programs. CtBP1 participates in epigenetic control through interactions with histone deacetylases and other remodeling factors, influencing processes such as differentiation, apoptosis, and metabolic adaptation. As a NAD(H)-sensitive coregulator, CtBP1 integrates cellular redox state with transcriptional outputs and contributes to pathways including Wnt/β-catenin and TGF-β–associated transcriptional networks. Altered CTBP1 activity has been associated with dysregulated transcriptional repression in cancer biology and neurodevelopmental phenotypes, supporting its study in gene regulatory circuitry and disease-relevant cell states.
CtBP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.