CRM1 Double Nickase Plasmid (h): sc-400348-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRM1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CRM1 Double Nickase Plasmid (h) and CRM1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XPO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRM1 Antibody (C-1): sc-74454
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRM1 Double Nickase Plasmid (h) | sc-400348-NIC | 20 µg | $410.00 | |||
CRM1 Double Nickase Plasmid (h2) | sc-400348-NIC-2 | 20 µg | $410.00 |
XPO1 encodes CRM1, a RanGTP-dependent nuclear export receptor that recognizes leucine-rich nuclear export signals and mediates directional transport of proteins and select RNAs through the nuclear pore complex. CRM1-dependent export governs the subcellular localization of key regulators of transcription, cell-cycle progression, stress responses, and innate immune signaling, integrating with Ran cycle dynamics and nuclear transport pathways. Dysregulated CRM1 activity perturbs nuclear–cytoplasmic trafficking of tumor suppressors and signaling factors, and altered XPO1/CRM1 function has been associated with oncogenic programs, viral host–pathogen interactions, and neurodegenerative disease mechanisms. These features make XPO1 a central node for studying nucleocytoplasmic transport control and its downstream effects on gene expression and proteostasis.
CRM1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XPO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XPO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XPO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XPO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.