Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRIK Double Nickase Plasmid (m): sc-419668-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRIK Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CRIK Double Nickase Plasmid (m) and CRIK Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cit. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRIK Antibody (E-6): sc-390437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRIK Double Nickase Plasmid (m) | sc-419668-NIC | 20 µg | $410.00 | |||
CRIK Double Nickase Plasmid (m2) | sc-419668-NIC-2 | 20 µg | $410.00 |
Mouse Cit encodes citron Rho-interacting kinase (CRIK), a serine/threonine kinase that functions downstream of RhoA to coordinate actin cytoskeleton remodeling during cytokinesis. CRIK localizes to the cleavage furrow and midbody, where it supports contractile ring organization and abscission through regulation of myosin and other cytoskeletal effectors. This activity links Cit to cell-cycle progression, mitotic fidelity, and tissue homeostasis in proliferative compartments. Dysregulated cytokinesis and cytoskeletal control associated with altered CRIK function is relevant to studies of genome instability, aneuploidy, and developmental phenotypes in mouse models.
CRIK Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cit locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cit. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cit function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cit-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.