Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRF CRISPR/Cas9 KO Plasmid (h): sc-400770
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRF Antibody (2B11): sc-293187
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRF CRISPR/Cas9 KO Plasmid (h) | sc-400770 | 20 µg | $397.00 |
Overview
Corticotropin-releasing hormone (CRH) encodes the neuropeptide CRF, a central regulator of the hypothalamic–pituitary–adrenal (HPA) axis that coordinates endocrine, autonomic, and behavioral responses to physiological stress. Upon secretion, CRF signals primarily through CRHR1/CRHR2 to activate Gs–adenylyl cyclase–cAMP/PKA pathways, modulating CREB-dependent transcription and downstream neuroendocrine outputs including ACTH release. CRH/CRF activity also interfaces with inflammatory and metabolic signaling, shaping cytokine production and energy homeostasis across brain and peripheral tissues. Dysregulated CRH expression or CRF receptor signaling has been associated with stress-related neuropsychiatric phenotypes and with inflammatory and gastrointestinal disorders, making it relevant for mechanistic studies of stress–immune crosstalk.
CRF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CRH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CRH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CRH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRF protein expression.
This CRISPR knockout system enables efficient generation of CRH-deficient cell models for investigation of CRF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CRH exon(s) critical for CRF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CRH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRF CRISPR/Cas9 KO Plasmid (h) and CRF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CRH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRF HDR Plasmid (h) and CRF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CRH homology arms to support homology-directed repair at defined CRH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.