COX5b Double Nickase Plasmid (h): sc-405159-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COX5b Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COX5b Double Nickase Plasmid (h) and COX5b Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COX5B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COX5b Antibody (C-5): sc-374416
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COX5b Double Nickase Plasmid (h) | sc-405159-NIC | 20 µg | $410.00 | |||
COX5b Double Nickase Plasmid (h2) | sc-405159-NIC-2 | 20 µg | $410.00 |
COX5B encodes cytochrome c oxidase subunit 5B (COX5b), a nuclear-encoded component of mitochondrial complex IV that helps regulate electron transfer from cytochrome c to oxygen and supports efficient oxidative phosphorylation. By contributing to proton pumping and mitochondrial membrane potential, COX5b influences ATP production, reactive oxygen species balance, and metabolic adaptation under varying oxygen and nutrient conditions. COX5B function intersects with mitochondrial biogenesis and quality control pathways, including respiratory chain assembly and mitophagy, which are central to cellular stress responses. Altered complex IV activity and COX5B-associated mitochondrial dysfunction have been linked to bioenergetic defects observed across multiple disease contexts, including neuromuscular and neurodegenerative phenotypes, where mitochondrial performance constrains cell survival and differentiation.
COX5b Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COX5B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COX5B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COX5B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COX5B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.