Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COX4 Double Nickase Plasmid (h): sc-400616-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COX4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COX4 Double Nickase Plasmid (h) and COX4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COX4I1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COX4 Antibody (F-8): sc-376731
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COX4 Double Nickase Plasmid (h) | sc-400616-NIC | 20 µg | $410.00 | |||
COX4 Double Nickase Plasmid (h2) | sc-400616-NIC-2 | 20 µg | $410.00 |
COX4I1 encodes cytochrome c oxidase subunit IV isoform 1 (COX4), a nuclear-encoded regulatory component of mitochondrial complex IV that tunes electron transfer from cytochrome c to oxygen and supports proton pumping for oxidative phosphorylation. COX4 integrates mitochondrial respiration with cellular energy homeostasis and redox balance, influencing ATP production, reactive oxygen species signaling, and metabolic adaptation to nutrient and oxygen availability. Perturbation of complex IV function is linked to mitochondrial dysfunction phenotypes, including impaired bioenergetics and altered stress responses, and is frequently examined in contexts such as neurodegeneration, cardiometabolic pathology, and tumor metabolism. COX4I1 is therefore a useful target for dissecting mitochondrial respiratory control and its coupling to cell survival, proliferation, and differentiation programs.
COX4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COX4I1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COX4I1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COX4I1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COX4I1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.