Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COL9A2 CRISPR/Cas9 KO Plasmid (h): sc-405890
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL9A2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL9A2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL9A2 Antibody (H-8): sc-398130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL9A2 CRISPR/Cas9 KO Plasmid (h) | sc-405890 | 20 µg | $397.00 |
Overview
COL9A2 encodes the α2 chain of type IX collagen, a FACIT collagen that associates with type II collagen fibrils to stabilize and organize the extracellular matrix of hyaline cartilage. By contributing to fibrillogenesis and matrix–cell interactions, COL9A2 supports chondrocyte function, cartilage tensile strength, and mechanotransduction processes central to skeletal development and joint homeostasis. Perturbation of COL9A2 expression or structure is linked to heritable cartilage disorders and has been studied in the context of early-onset degenerative joint phenotypes and intervertebral disc pathology. As a matrix component, COL9A2 connects extracellular matrix remodeling with pathways governing cartilage integrity, including collagen assembly, proteoglycan organization, and stress-responsive signaling in chondrocytes.
COL9A2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COL9A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COL9A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COL9A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL9A2 protein expression.
This CRISPR knockout system enables efficient generation of COL9A2-deficient cell models for investigation of COL9A2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COL9A2 exon(s) critical for COL9A2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COL9A2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL9A2 CRISPR/Cas9 KO Plasmid (h) and COL9A2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COL9A2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL9A2 HDR Plasmid (h) and COL9A2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COL9A2 homology arms to support homology-directed repair at defined COL9A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.