COL4A2 Double Nickase Plasmid (h): sc-401462-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL4A2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL4A2 Double Nickase Plasmid (h) and COL4A2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL4A2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL4A2 Double Nickase Plasmid (h) | sc-401462-NIC | 20 µg | $410.00 | |||
COL4A2 Double Nickase Plasmid (h2) | sc-401462-NIC-2 | 20 µg | $410.00 |
COL4A2 encodes the alpha-2 chain of type IV collagen, a principal structural component of basement membranes that assembles into COL4A1/COL4A2 heterotrimers and higher-order collagen IV networks. This extracellular matrix scaffold supports tissue architecture, regulates permeability, and organizes signaling microenvironments that influence cell adhesion, migration, and differentiation through integrin- and matrix-dependent pathways. COL4A2 is central to basement membrane biogenesis and vascular integrity, and variants are linked to small-vessel and basement membrane disorders with prominent cerebrovascular and ocular manifestations. As a matrix gene with broad developmental and tissue-specific roles, COL4A2 is frequently studied in endothelial biology, neurovascular unit function, and extracellular matrix remodeling.
COL4A2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL4A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL4A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL4A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL4A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.