COL4A1 Double Nickase Plasmid (h): sc-401792-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL4A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL4A1 Double Nickase Plasmid (h) and COL4A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL4A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL4A1 Antibody (5E10): sc-517572
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL4A1 Double Nickase Plasmid (h) | sc-401792-NIC | 20 µg | $410.00 | |||
COL4A1 Double Nickase Plasmid (h2) | sc-401792-NIC-2 | 20 µg | $410.00 |
COL4A1 encodes the alpha-1 chain of type IV collagen, a core structural component of basement membranes that assembles into collagen IV networks with COL4A2 to support tissue integrity and microvascular stability. This extracellular matrix scaffold helps organize cell–matrix interactions that shape epithelial and endothelial polarity, regulate permeability, and influence mechanotransduction through integrin-linked signaling. COL4A1 function intersects with basement membrane assembly, extracellular matrix organization, and angiogenic programs that affect vessel maturation and tissue homeostasis. Dysregulation or pathogenic variation in COL4A1 is associated with small-vessel pathology and multisystem basement membrane defects, making it a relevant target for studying vascular biology, barrier function, and matrix-dependent cellular phenotypes.
COL4A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL4A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL4A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL4A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL4A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.