COL1A2 Double Nickase Plasmid (h): sc-400598-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL1A2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL1A2 Double Nickase Plasmid (h) and COL1A2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL1A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pro-COL1A2 Antibody (D-6): sc-166572
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL1A2 Double Nickase Plasmid (h) | sc-400598-NIC | 20 µg | $410.00 | |||
COL1A2 Double Nickase Plasmid (h2) | sc-400598-NIC-2 | 20 µg | $410.00 |
COL1A2 encodes the pro-α2 chain of type I collagen, a principal fibrillar collagen that assembles into heterotrimers with COL1A1 to form the structural scaffold of extracellular matrix in bone, tendon, dermis, and other connective tissues. Type I collagen biosynthesis and maturation involve procollagen processing, triple-helix formation, secretion, and extracellular fibrillogenesis, coordinating with integrin-mediated adhesion, mechanotransduction, and matrix remodeling pathways. Altered COL1A2 expression or sequence can perturb collagen fiber organization and matrix stiffness, disrupting tissue integrity and influencing cell–matrix signaling in musculoskeletal and fibrotic contexts. As a core ECM component, COL1A2 is frequently studied in osteoblast differentiation, wound repair, stromal biology, and matrix-driven regulation of growth factor availability.
COL1A2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL1A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL1A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL1A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL1A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.