COL11A2 CRISPR/Cas9 KO Plasmid (h): sc-406243
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL11A2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL11A2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL11A2 CRISPR/Cas9 KO Plasmid (h) | sc-406243 | 20 µg | $397.00 |
Overview
COL11A2 encodes the α2 chain of type XI collagen, a quantitatively minor but structurally important fibrillar collagen that integrates with type II collagen to regulate collagen fibril diameter and extracellular matrix organization, particularly in cartilage. Its expression supports chondrocyte differentiation, tissue biomechanics, and matrix assembly during skeletal development, influencing processes such as collagen fibrillogenesis and pericellular matrix formation. Disruption of COL11A2 has been linked to heritable skeletal dysplasias and sensorineural hearing loss, reflecting its roles in cartilage integrity and inner ear extracellular matrix architecture. As a matrix gene, COL11A2 is also used to study ECM-dependent signaling and cell–matrix interactions that modulate cellular phenotype.
COL11A2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COL11A2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COL11A2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COL11A2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL11A2 protein expression.
This CRISPR knockout system enables efficient generation of COL11A2-deficient cell models for investigation of COL11A2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COL11A2 exon(s) critical for COL11A2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COL11A2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL11A2 CRISPR/Cas9 KO Plasmid (h) and COL11A2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COL11A2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL11A2 HDR Plasmid (h) and COL11A2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COL11A2 homology arms to support homology-directed repair at defined COL11A2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.