COL11A1 Double Nickase Plasmid (h): sc-403512-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL11A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL11A1 Double Nickase Plasmid (h) and COL11A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL11A1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL11A1 Double Nickase Plasmid (h) | sc-403512-NIC | 20 µg | $410.00 | |||
COL11A1 Double Nickase Plasmid (h2) | sc-403512-NIC-2 | 20 µg | $410.00 |
COL11A1 encodes the α1 chain of type XI collagen, a quantitatively minor but functionally important fibrillar collagen that co-assembles with type II collagen to regulate collagen fibril diameter, spacing, and tensile properties in the extracellular matrix. It is highly relevant to cartilage and connective tissue biology, where it contributes to matrix organization, chondrocyte differentiation, and mechanotransduction-related signaling. Altered COL11A1 expression or sequence has been linked to extracellular matrix remodeling phenotypes and connective tissue disorders, and it is frequently studied in the context of stromal signatures and tumor microenvironment desmoplasia. As an ECM structural gene, COL11A1 provides a tractable entry point for investigating matrix-dependent pathways, including integrin-mediated adhesion, focal adhesion dynamics, and TGF-β–associated remodeling programs.
COL11A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL11A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL11A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL11A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL11A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.