Clusterin CRISPR/Cas9 KO Plasmid (m): sc-419695
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clusterin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Clusterin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Clusterin-α Antibody (A-11): sc-166831
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clusterin CRISPR/Cas9 KO Plasmid (m) | sc-419695 | 20 µg | $397.00 |
Overview
Mouse Clu encodes clusterin, a secreted and extracellular matrix–associated glycoprotein that functions as a molecular chaperone and lipid-binding factor implicated in proteostasis, complement regulation, and clearance of cellular debris. Clusterin participates in stress-responsive processes including apoptosis modulation, oxidative stress adaptation, and membrane remodeling, and it has been linked to pathways governing inflammation and extracellular homeostasis. In the nervous system and other tissues, CLU is frequently studied for roles in protein aggregation dynamics and glial or immune signaling. Dysregulated CLU expression or function has been associated with neurodegeneration, metabolic stress, and tumor biology, making it a useful node for mechanistic studies of tissue injury and chronic inflammatory states.
Clusterin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Clu gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Clu together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Clu open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Clusterin protein expression.
This CRISPR knockout system enables efficient generation of Clu-deficient cell models for investigation of Clusterin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Clu exon(s) critical for Clusterin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Clu genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Clusterin CRISPR/Cas9 KO Plasmid (m) and Clusterin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Clu locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Clusterin HDR Plasmid (m) and Clusterin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Clu homology arms to support homology-directed repair at defined Clu target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.