Clathrin Heavy Chain/CLTC Antibody (E-11): sc-271253

Clathrin HC Antibody (E-11) is a mouse monoclonal IgG1 antibody that detects Clathrin HC in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Clathrin HC plays a crucial role in endocytosis, facilitating the formation of clathrin-coated vesicles that transport molecules into cells. Clathrin HC is primarily located in the cytoplasm, where anti-Clathrin HC antibody (E-11) can detect basket-like structures around budding vesicles, ensuring efficient internalization of receptor proteins and other cargo. Proper functioning of Clathrin HC maintains cellular homeostasis and regulates signaling pathways, as disruptions can lead to various diseases, including neurodegenerative disorders and cancer. Clathrin HC is composed of three heavy chains and three light chains that non-covalently associate to form a triskelion structure, with the proximal segment of the heavy chain being essential for binding to light chains, thereby stabilizing the overall structure. Clathrin HC monoclonal antibody (E-11) serves as an invaluable tool for researchers studying endocytic pathways and their implications in health and disease.

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Clathrin Heavy Chain/CLTC Antibody (E-11) References:

1. T cell receptor engagement leads to phosphorylation of clathrin heavy chain during receptor internalization.  |  Crotzer, VL., et al. 2004. J Exp Med. 199: 981-91. PMID: 15067034
2. The Tom1L1-clathrin heavy chain complex regulates membrane partitioning of the tyrosine kinase Src required for mitogenic and transforming activities.  |  Collin, G., et al. 2007. Mol Cell Biol. 27: 7631-40. PMID: 17785434
3. Introns outperform exons in analyses of basal avian phylogeny using clathrin heavy chain genes.  |  Chojnowski, JL., et al. 2008. Gene. 410: 89-96. PMID: 18191344
4. Identification of novel immunohistochemical tumor markers for primary hepatocellular carcinoma; clathrin heavy chain and formiminotransferase cyclodeaminase.  |  Seimiya, M., et al. 2008. Hepatology. 48: 519-30. PMID: 18571811
5. Identification of a function-specific mutation of clathrin heavy chain (CHC) required for p53 transactivation.  |  Ohata, H., et al. 2009. J Mol Biol. 394: 460-71. PMID: 19766654
6. Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling.  |  Li, X., et al. 2010. EMBO J. 29: 992-1006. PMID: 20111007
7. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78.  |  Kitahara, H., et al. 2011. Mol Cancer Res. 9: 688-701. PMID: 21571834
8. Characterization of a gene encoding clathrin heavy chain in maize up-regulated by salicylic acid, abscisic acid and high boron supply.  |  Zeng, MH., et al. 2013. Int J Mol Sci. 14: 15179-98. PMID: 23880865
9. Nuclear Magnetic Resonance Structural Mapping Reveals Promiscuous Interactions between Clathrin-Box Motif Sequences and the N-Terminal Domain of the Clathrin Heavy Chain.  |  Zhuo, Y., et al. 2015. Biochemistry. 54: 2571-80. PMID: 25844500
10. Isolation of a new clathrin heavy chain gene with muscle-specific expression from the region commonly deleted in velo-cardio-facial syndrome.  |  Sirotkin, H., et al. 1996. Hum Mol Genet. 5: 617-24. PMID: 8733128

The preferred Clathrin Heavy Chain/CLTC antibody is Clathrin Heavy Chain/CLTC Antibody (TD.1): sc-12734.