Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CHMP1B CRISPR/Cas9 KO Plasmid (h): sc-406531
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHMP1B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHMP1B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CHMP1B Antibody (D-10): sc-514013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHMP1B CRISPR/Cas9 KO Plasmid (h) | sc-406531 | 20 µg | $397.00 |
Overview
CHMP1B (charged multivesicular body protein 1B) is a core component of the ESCRT-III machinery that drives membrane remodeling events required for endosomal sorting and multivesicular body biogenesis. Through ESCRT-dependent pathways, CHMP1B contributes to receptor downregulation, lysosomal trafficking, autophagy-related membrane dynamics, and completion of cytokinetic abscission. Proper CHMP1B activity helps maintain proteostasis and signaling homeostasis by governing the fate of ubiquitinated cargo and membrane proteins. Dysregulation of ESCRT-III function, including altered CHMP1B-dependent trafficking, is frequently studied in contexts of neurodegenerative processes, viral budding, and cancer-associated changes in growth factor receptor signaling.
CHMP1B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CHMP1B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CHMP1B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CHMP1B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHMP1B protein expression.
This CRISPR knockout system enables efficient generation of CHMP1B-deficient cell models for investigation of CHMP1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CHMP1B exon(s) critical for CHMP1B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CHMP1B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHMP1B CRISPR/Cas9 KO Plasmid (h) and CHMP1B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CHMP1B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHMP1B HDR Plasmid (h) and CHMP1B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CHMP1B homology arms to support homology-directed repair at defined CHMP1B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.