Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CHMP1A CRISPR/Cas9 KO Plasmid (h): sc-406556
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHMP1A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHMP1A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CHMP1A Antibody (B-5): sc-271617
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHMP1A CRISPR/Cas9 KO Plasmid (h) | sc-406556 | 20 µg | $397.00 |
Overview
CHMP1A (charged multivesicular body protein 1A) is a core component of the ESCRT-III machinery that remodels membranes to drive multivesicular body biogenesis and endosomal cargo sorting toward lysosomal degradation. Through ESCRT-dependent processes, CHMP1A contributes to receptor downregulation, membrane scission events, and broader regulation of proteostasis, cytokinesis, and nuclear envelope dynamics. Disruption of CHMP1A-associated endolysosomal trafficking can alter signaling pathway duration and cellular homeostasis, linking ESCRT dysfunction to neurodevelopmental phenotypes and other disorders characterized by impaired membrane trafficking. As a human gene, CHMP1A is frequently studied in contexts where endosome maturation and vesicle-mediated signaling control are central to disease-relevant cellular states.
CHMP1A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CHMP1A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CHMP1A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CHMP1A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHMP1A protein expression.
This CRISPR knockout system enables efficient generation of CHMP1A-deficient cell models for investigation of CHMP1A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CHMP1A exon(s) critical for CHMP1A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CHMP1A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHMP1A CRISPR/Cas9 KO Plasmid (h) and CHMP1A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CHMP1A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHMP1A HDR Plasmid (h) and CHMP1A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CHMP1A homology arms to support homology-directed repair at defined CHMP1A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.