CHD1 Double Nickase Plasmid (h): sc-404299-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CHD1 Double Nickase Plasmid (h) and CHD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CHD1 Antibody (C-8): sc-271626
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHD1 Double Nickase Plasmid (h) | sc-404299-NIC | 20 µg | $410.00 | |||
CHD1 Double Nickase Plasmid (h2) | sc-404299-NIC-2 | 20 µg | $410.00 |
CHD1 (chromodomain helicase DNA binding protein 1) is an ATP-dependent chromatin remodeler that recognizes methylated histone marks via its chromodomains and repositions nucleosomes to shape chromatin accessibility. It supports transcriptional regulation, RNA polymerase II elongation, and maintenance of open chromatin at active promoters and gene bodies, linking it to processes such as DNA replication, DNA damage response, and genome stability. CHD1 function intersects with chromatin organization and epigenetic control pathways that influence cell identity and differentiation programs. Altered CHD1 activity or genomic status has been associated with transcriptional dysregulation and genome instability phenotypes relevant to cancer biology and related chromatin-driven disease mechanisms.
CHD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.