Anti-Cdc28 Anticuerpo G-7 es un monoclonal de ratón IgG1 (kappa light chain) Cdc28 Anticuerpo proporcionado como 200 µg/ml
específico para un epítopo localizado entre los amino ácidos 241-259 cerca del C-terminus de oe orgiendf Saccharomyces cerevisiaeorigin
Cdc28 Anticuerpo (G-7) es recomendado para detectar Cdc28 de y origen, mediante WB, IP, IF y ELISA
Cdc28 Anticuerpo (G-7) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cdc28 (G-7): sc-515762.
m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc28 Antibody (G-7) for WB applications. These reagents are now offered in bundles with Cdc28 Antibody (G-7) (see ordering information below).
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Cdc28 Antibody (G-7) is a high quality monoclonal Cdc28 antibody (also designated SRM5 antibody and Cyclin-dependent kinase 1 antibody) suitable for the detection of the Cdc28 protein of Saccharomyces cerevisiae origin. Cdc28 Antibody (G-7) is available as both the non-conjugated anti-Cdc28 antibody form, as well as multiple conjugated forms of anti-Cdc28 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28. Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family, including Cln1, Cln2 and Cln3 (also designated DAF1 or WHI1). The G2 to M phase requires the M phase cyclins, Clb1 (also designated Scb1) and Clb2, as well as the G2 cyclins, Clb3 and Clb4. The S phase cyclins Clb5 and Clb6 coordinate DNA replication with cytokinesis. Expression of the cyclins is controlled by Ubc9 and Cdc34 (also designated Udc3 or Dna6) via ubiquitin-mediated proteolysis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for Cdc28 (G-7): sc-515762 monoclonal antibody?
Preguntado por: Randy McDonald
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link:
If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
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