Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD88 Double Nickase Plasmid (m): sc-419395-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD88 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD88 Double Nickase Plasmid (m) and CD88 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting C5ar1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD88 Antibody (R63): sc-53797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD88 Double Nickase Plasmid (m) | sc-419395-NIC | 20 µg | $410.00 | |||
CD88 Double Nickase Plasmid (m2) | sc-419395-NIC-2 | 20 µg | $410.00 |
C5ar1 encodes CD88, the canonical G protein–coupled receptor for the complement anaphylatoxin C5a, linking complement activation to leukocyte chemotaxis, degranulation, oxidative burst, and cytokine production. CD88 signaling engages pathways including PI3K–AKT, MAPK/ERK, PLCβ–Ca²⁺ flux, and NF-κB to shape innate immune activation and inflammatory cell trafficking. In mouse models, C5ar1 activity is frequently studied in contexts of infection, sterile inflammation, autoimmunity, neuroinflammation, and tissue injury where complement-driven responses influence disease-associated phenotypes. Because CD88 is expressed across myeloid populations and some non-hematopoietic cells, it serves as a key node for dissecting complement–immune crosstalk in complex microenvironments.
CD88 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the C5ar1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C5ar1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C5ar1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C5ar1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.