CD74 Anticuerpo (2X31) recomendado para detectarCD74 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y FCM

CD74 Anticuerpo (2X31): sc-70781

CD74 Anticuerpo (2X31) clasificado 3.0 de 5 por 2.
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Ficha técnica

    • CD74 Anticuerpo (2X31) es un monoclonal de ratón IgG1 κ, ver las 1 publicaciones, proporcionado como 200 µg/ml
    • raised against SU-DHL-4 lymphoma cells
    • recomendado para detectarCD74 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y FCM
    • Ver CD74 (LN-2): sc-6262 para CD74 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.

May I know which site/ epitope of CD74 that CD74 (2X31): sc70781 recognises/ binds? I am curious about whether this antibody can detect all forms of CD74 (as I created various CD74 mutants).

Preguntado por: Lynn93
Thank you for the question. This CD74 specific antibody was raised against SU-DHL-4 lymphoma cells. The exact binding epitope has not been determined exactly, yet. If you are working with CD74 mutants you might also want to check other available CD74 antibodies. We offer two antibodies raised against specific peptides/ rec. protein fragments: sc-47742 was raised against aas 12-28 (h) sc-166047 was raised against aas 7-296 (h) https://www.scbt.com/scbt/browse/cd74-Antik%C3%B6rper/_/N-6thh37
Respondida por: Technical Support Europe
Fecha de publicación: 2018-01-11

Can CD74 (2X31): sc-70781 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Preguntado por: jerojero
Thank you for your question. Yes, CD74 (2X31): sc-70781 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-28
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Rated 3 de 5 por de Robust bands seen in WB using NAMALWA Robust bands seen in WB using NAMALWA, IB4, J774.A1 and BJAB whole cell lysates. -SCBT QC
Fecha de publicación: 2015-05-24
Rated 3 de 5 por de Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Fecha de publicación: 2013-06-01
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