Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD59 Double Nickase Plasmid (h): sc-417670-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD59 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD59 Double Nickase Plasmid (h) and CD59 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD59. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD59 Antibody (H-7): sc-133170
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD59 Double Nickase Plasmid (h) | sc-417670-NIC | 20 µg | $410.00 | |||
CD59 Double Nickase Plasmid (h2) | sc-417670-NIC-2 | 20 µg | $410.00 |
CD59 (protectin) is a glycosylphosphatidylinositol-anchored membrane regulator of complement that inhibits assembly of the terminal membrane attack complex (C5b-9) on host cells. By restricting complement-mediated cytolysis and limiting bystander injury, CD59 helps maintain immune homeostasis at the cell surface and shapes inflammatory signaling in vascular, hematopoietic, and epithelial compartments. Altered CD59 expression or function is linked to dysregulated complement activation and contributes to disease mechanisms involving hemolysis, neuroinflammation, and tissue injury in complement-driven pathology. As a widely expressed surface protein, CD59 is also used to study membrane microdomain biology, immune evasion, and crosstalk between innate immunity and cell survival programs.
CD59 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD59 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD59. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD59 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD59-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.