Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD42c Double Nickase Plasmid (h): sc-403712-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD42c Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD42c Double Nickase Plasmid (h) and CD42c Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GP1BB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD42c Antibody (F-11): sc-377129
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD42c Double Nickase Plasmid (h) | sc-403712-NIC | 20 µg | $410.00 | |||
CD42c Double Nickase Plasmid (h2) | sc-403712-NIC-2 | 20 µg | $410.00 |
GP1BB encodes the platelet glycoprotein Ib beta chain (CD42c), an essential component of the GPIb-IX-V receptor complex that mediates platelet tethering and adhesion to von Willebrand factor under high shear stress. Through coordination with cytoskeletal adaptor proteins and signaling nodes such as Src family kinases and PI3K-dependent pathways, CD42c contributes to platelet activation, procoagulant responses, and thrombus formation. Genetic disruption or altered expression of GP1BB perturbs receptor surface assembly and platelet function, linking this axis to inherited platelet disorders characterized by macrothrombocytopenia and bleeding phenotypes. As a result, GP1BB is widely studied in platelet biogenesis, hemostasis, and mechanotransduction models relevant to vascular biology and inflammatory thrombosis research.
CD42c Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GP1BB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GP1BB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GP1BB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GP1BB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.