Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD2 Double Nickase Plasmid (h): sc-402105-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD2 Double Nickase Plasmid (h) and CD2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD2 Antibody (MT910): sc-19638
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD2 Double Nickase Plasmid (h) | sc-402105-NIC | 20 µg | $410.00 | |||
CD2 Double Nickase Plasmid (h2) | sc-402105-NIC-2 | 20 µg | $410.00 |
CD2 encodes a cell-surface adhesion and signaling receptor of the immunoglobulin superfamily that is highly expressed on T cells and NK cells, where it binds ligands such as CD58 to stabilize immune synapse formation. CD2 co-stimulates T cell receptor signaling and shapes downstream pathways controlling calcium flux, MAPK activation, cytoskeletal remodeling, and cytokine production. Through these functions, CD2 contributes to lymphocyte development, antigen-driven activation, and cell–cell communication within inflamed tissues. Dysregulated CD2-mediated signaling and adhesion have been implicated in immune dysfunction contexts, including autoimmunity and hematologic malignancy biology, making it a relevant node for mechanistic immunology studies.
CD2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.