Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD14 Double Nickase Plasmid (m): sc-419531-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD14 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD14 Double Nickase Plasmid (m) and CD14 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cd14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD14 Antibody (H-4): sc-515785
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD14 Double Nickase Plasmid (m) | sc-419531-NIC | 20 µg | $410.00 | |||
CD14 Double Nickase Plasmid (m2) | sc-419531-NIC-2 | 20 µg | $410.00 |
Mouse Cd14 encodes CD14, a glycosylphosphatidylinositol-anchored co-receptor that binds bacterial lipopolysaccharide and other pathogen-associated ligands to facilitate innate immune recognition. CD14 cooperates with the TLR4–MD-2 complex to amplify MyD88- and TRIF-dependent signaling, promoting NF-κB and IRF activation and downstream cytokine and type I interferon responses. It also contributes to ligand internalization, endosomal trafficking, and modulation of inflammasome-adjacent inflammatory programs in myeloid cells. Dysregulated CD14 signaling is linked to aberrant inflammatory states and is widely studied in models of sepsis-like responses, metabolic inflammation, and neuroinflammatory processes.
CD14 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cd14 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cd14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cd14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cd14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.