Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD14 CRISPR Activation Plasmid (h): sc-400344-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD14 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- CD14 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by CD14 CRISPR Activation Plasmid (h) and CD14 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CD14 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD14 Antibody (5A3B11B5): sc-58951
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD14 CRISPR Activation Plasmid (h) | sc-400344-ACT | 20 µg | $397.00 | |||
CD14 CRISPR Activation Plasmid (h2) | sc-400344-ACT-2 | 20 µg | $397.00 |
Human CD14 encodes a glycosylphosphatidylinositol (GPI)-anchored co-receptor predominantly expressed on monocytes, macrophages, and neutrophils that enhances innate immune recognition of bacterial lipopolysaccharide and other pathogen-associated ligands. CD14 cooperates with TLR4–MD-2 and LBP to promote MyD88- and TRIF-dependent signaling, driving NF-κB and IRF-mediated inflammatory transcriptional programs and cytokine production. As a regulator of endotoxin sensing and myeloid activation states, altered CD14 expression and signaling dynamics are frequently studied in the context of infection biology, chronic inflammatory disorders, and immune dysregulation. CD14 is also used as a marker and functional modulator in analyses of monocyte-to-macrophage differentiation, polarization, and microenvironmental responses.
CD14 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CD14 expression without altering the underlying DNA sequence.
CD14 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CD14 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CD14 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous CD14 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CD14 locus and enabling the study of CD14-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of CD14 pathway restoration in tumor cells with silenced or reduced CD14 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.