Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
caveolin-3 CRISPR/Cas9 KO Plasmid (h): sc-400569
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caveolin-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the caveolin-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caveolin-3 Antibody (A-3): sc-5310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caveolin-3 CRISPR/Cas9 KO Plasmid (h) | sc-400569 | 20 µg | $397.00 |
Overview
CAV3 encodes caveolin-3, a muscle-enriched structural component of caveolae that organizes membrane microdomains and scaffolds signaling complexes at the sarcolemma. Caveolin-3 contributes to mechanoprotection and membrane trafficking and modulates pathways involving GPCR signaling, insulin receptor signaling, ion channel regulation, and nitric oxide signaling through compartmentalization of receptors and kinases. In striated muscle, it supports T-tubule organization, excitation–contraction coupling, and stability of the dystrophin-associated protein complex. Altered CAV3 function or expression is associated with caveolinopathies affecting skeletal and cardiac muscle, including dystrophic phenotypes, cardiomyopathy, and arrhythmia-related mechanisms, making it relevant for studies of membrane signaling and muscle integrity.
caveolin-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CAV3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CAV3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CAV3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish caveolin-3 protein expression.
This CRISPR knockout system enables efficient generation of CAV3-deficient cell models for investigation of caveolin-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CAV3 exon(s) critical for caveolin-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CAV3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by caveolin-3 CRISPR/Cas9 KO Plasmid (h) and caveolin-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CAV3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by caveolin-3 HDR Plasmid (h) and caveolin-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CAV3 homology arms to support homology-directed repair at defined CAV3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.