caveolin-2 Double Nickase Plasmid (h): sc-402547-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caveolin-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- caveolin-2 Double Nickase Plasmid (h) and caveolin-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAV2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caveolin-2 Double Nickase Plasmid (h) | sc-402547-NIC | 20 µg | $410.00 | |||
caveolin-2 Double Nickase Plasmid (h2) | sc-402547-NIC-2 | 20 µg | $410.00 |
Human CAV2 encodes caveolin-2, an integral membrane scaffolding protein enriched in caveolae that helps organize lipid raft microdomains and regulate membrane curvature, endocytosis, and cholesterol homeostasis. Caveolin-2 cooperates with caveolin-1 to influence caveolae assembly and modulate signaling nodes such as receptor tyrosine kinases, eNOS/NO signaling, and downstream MAPK and PI3K/AKT pathway dynamics in a cell-type-dependent manner. Through effects on membrane trafficking, adhesion, and mechanotransduction, CAV2 has been associated with vascular and pulmonary biology, metabolic regulation, and altered signaling states observed across multiple disease contexts, including cancer-related phenotypes. As a marker and regulator of caveolar function, CAV2 is frequently studied in endothelial cells, fibroblasts, and epithelial systems to probe compartmentalized signaling and membrane-dependent stress responses.
caveolin-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAV2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAV2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAV2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAV2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.