CALM Antibody (D-8): sc-166522

CALM Antibody (D-8) is a mouse monoclonal IgG1 antibody that detects CALM in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CALM, or clathrin assembly protein lymphoid myeloid leukemia, plays a crucial role in clathrin-mediated endocytosis, which is essential for cellular uptake of nutrients and signaling molecules. CALM is primarily located in the cytoplasm and participates in clathrin-coated vesicle assembly, facilitating transport of various cargoes from the plasma membrane and trans-Golgi network. CALM′s cellular location enables interaction with clathrin and other adaptor proteins, such as AP180, to form functional clathrin-coated pits that initiate endocytosis. CALM′s homologous relationship with AP180 suggests shared functions in synaptic vesicle recycling, highlighting CALM′s significance in maintaining cellular homeostasis and communication. Anti-CALM antibody (D-8) serves as an invaluable tool for researchers studying endocytic pathways and molecular mechanisms underlying cellular transport processes.

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CALM Antibody (D-8) References:

1. Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic.  |  Tebar, F., et al. 1999. Mol Biol Cell. 10: 2687-702. PMID: 10436022
2. Purification of clathrin assembly protein from rat liver.  |  Kim, HL. and Kim, JA. 2000. Exp Mol Med. 32: 222-6. PMID: 11190274
3. A novel chromosomal inversion at 11q23 in infant acute myeloid leukemia fuses MLL to CALM, a gene that encodes a clathrin assembly protein.  |  Wechsler, DS., et al. 2003. Genes Chromosomes Cancer. 36: 26-36. PMID: 12461747
4. Effect of clathrin assembly lymphoid myeloid leukemia protein depletion on clathrin coat formation.  |  Meyerholz, A., et al. 2005. Traffic. 6: 1225-34. PMID: 16262731
5. Clathrin assembly lymphoid myeloid leukemia-AF10-positive acute leukemias: a report of 2 cases with a review of the literature.  |  Huh, JY., et al. 2010. Korean J Lab Med. 30: 117-21. PMID: 20445327
6. SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors clathrin assembly lymphoid myeloid leukemia (CALM) and AP180 at synapses.  |  Koo, SJ., et al. 2011. Proc Natl Acad Sci U S A. 108: 13540-5. PMID: 21808019
7. The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice.  |  Suzuki, M., et al. 2012. PLoS One. 7: e31854. PMID: 22363754
8. Role of phosphatidylinositol clathrin assembly lymphoid-myeloid leukemia (PICALM) in intracellular amyloid precursor protein (APP) processing and amyloid plaque pathogenesis.  |  Xiao, Q., et al. 2012. J Biol Chem. 287: 21279-89. PMID: 22539346
9. The ∼ 16 kDa C-terminal sequence of clathrin assembly protein AP180 is essential for efficient clathrin binding.  |  Chan, LS., et al. 2014. PLoS One. 9: e110557. PMID: 25329427
10. CALM supports clathrin-coated vesicle completion upon membrane tension increase.  |  Willy, NM., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34155137

The preferred CALM antibody is CALM Antibody (A-2): sc-271224.