C9orf169 CRISPR/Cas9 KO Plasmid (h): sc-406346
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C9orf169 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C9orf169 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C9orf169 CRISPR/Cas9 KO Plasmid (h) | sc-406346 | 20 µg | $397.00 |
Overview
CYSRT1 (also annotated as C9orf169) encodes a small protein with emerging roles in epithelial differentiation programs and barrier-associated biology, with reported enrichment in keratinizing tissues such as the upper aerodigestive tract. Although its molecular mechanism remains incompletely defined, expression patterns and co-regulation with cornified envelope and keratinization genes suggest involvement in terminal differentiation, cellular stress responses, and maintenance of epithelial homeostasis. Dysregulated CYSRT1/C9orf169 expression has been observed across multiple transcriptomic studies of squamous epithelia, supporting its use as a marker and functional node in pathways linked to proliferation–differentiation balance. These attributes make CYSRT1/C9orf169 relevant for investigating epithelial biology, inflammatory microenvironments, and context-dependent changes associated with squamous cell transformation.
C9orf169 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CYSRT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CYSRT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CYSRT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C9orf169 protein expression.
This CRISPR knockout system enables efficient generation of CYSRT1-deficient cell models for investigation of C9orf169 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CYSRT1 exon(s) critical for C9orf169 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CYSRT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C9orf169 CRISPR/Cas9 KO Plasmid (h) and C9orf169 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CYSRT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C9orf169 HDR Plasmid (h) and C9orf169 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CYSRT1 homology arms to support homology-directed repair at defined CYSRT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.