C17orf96 CRISPR/Cas9 KO Plasmid (h): sc-408662
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C17orf96 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C17orf96 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C17orf96 CRISPR/Cas9 KO Plasmid (h) | sc-408662 | 20 µg | $397.00 |
Overview
EPOP (C17orf96) encodes a chromatin-associated factor that functions as a component of Polycomb repressive complex 2 (PRC2), supporting epigenetic gene silencing through regulation of H3K27 methylation at target loci. By linking PRC2 to auxiliary proteins, C17orf96 helps coordinate transcriptional repression programs that shape cell identity, differentiation, and developmental gene expression. Dysregulation of PRC2-associated repressors and H3K27me3 landscapes is frequently implicated in oncogenic transcriptional states and other disorders with altered chromatin control. Accordingly, EPOP is studied in the context of chromatin remodeling, lineage specification, and aberrant repression of tumor suppressor and differentiation genes.
C17orf96 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EPOP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EPOP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EPOP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C17orf96 protein expression.
This CRISPR knockout system enables efficient generation of EPOP-deficient cell models for investigation of C17orf96 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EPOP exon(s) critical for C17orf96 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EPOP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C17orf96 CRISPR/Cas9 KO Plasmid (h) and C17orf96 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EPOP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C17orf96 HDR Plasmid (h) and C17orf96 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EPOP homology arms to support homology-directed repair at defined EPOP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.