Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
C/EBP α CRISPR/Cas9 KO Plasmid (m): sc-419621
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C/EBP α CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C/EBP α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: C/EBP α Antibody (D-5): sc-365318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C/EBP α CRISPR/Cas9 KO Plasmid (m) | sc-419621 | 20 µg | $397.00 |
Overview
Cebpa encodes the transcription factor C/EBPα, a basic leucine zipper regulator that coordinates lineage commitment and terminal differentiation in multiple tissues, with prominent roles in myeloid and adipocyte development. In hematopoietic cells, C/EBPα integrates signaling inputs to control cell-cycle exit and maturation programs by modulating transcriptional networks governing proliferation and differentiation. It interfaces with pathways involved in metabolic regulation, inflammatory gene expression, and chromatin remodeling, influencing cellular identity and stress responses. Dysregulated C/EBPα activity is frequently studied in the context of impaired differentiation, altered immune homeostasis, and hematologic disease mechanisms in mouse models.
C/EBP α CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cebpa gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cebpa together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cebpa open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C/EBP α protein expression.
This CRISPR knockout system enables efficient generation of Cebpa-deficient cell models for investigation of C/EBP α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cebpa exon(s) critical for C/EBP α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cebpa genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C/EBP α CRISPR/Cas9 KO Plasmid (m) and C/EBP α CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cebpa locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C/EBP α HDR Plasmid (m) and C/EBP α HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cebpa homology arms to support homology-directed repair at defined Cebpa target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.