Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BTBD4 CRISPR/Cas9 KO Plasmid (m): sc-428253
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BTBD4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BTBD4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BTBD4 Antibody (C-5): sc-390260
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD4 CRISPR/Cas9 KO Plasmid (m) | sc-428253 | 20 µg | $397.00 |
Overview
Zbtb46 encodes the BTBD4 protein, a BTB/POZ domain–containing factor implicated in transcriptional regulation programs that shape cell identity and differentiation states in mouse tissues. In immune biology, Zbtb46 expression is widely used as a marker of classical dendritic cell lineage, and its regulatory activity is studied in the context of antigen presentation, inflammatory signaling, and homeostatic immune surveillance. Altered Zbtb46-associated gene networks have been examined in models of tumor-associated immunity and inflammatory disease, where shifts in dendritic cell function can influence cytokine profiles and T cell priming. As a result, Zbtb46/BTBD4 perturbation is a useful approach for dissecting transcriptional circuits connecting differentiation, innate immune pathways, and microenvironmental responses.
BTBD4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zbtb46 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zbtb46 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zbtb46 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BTBD4 protein expression.
This CRISPR knockout system enables efficient generation of Zbtb46-deficient cell models for investigation of BTBD4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zbtb46 exon(s) critical for BTBD4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zbtb46 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BTBD4 CRISPR/Cas9 KO Plasmid (m) and BTBD4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zbtb46 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BTBD4 HDR Plasmid (m) and BTBD4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zbtb46 homology arms to support homology-directed repair at defined Zbtb46 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.