BrUTP (CAS 161848-60-8)
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5-Bromouridine 5′-triphosphate sodium (BrUTP) is a modified nucleotide that has found extensive application in scientific research for the investigation of RNA transcription and processing. As a fluorescently-labeled nucleotide, BrUTP can be integrated into RNA during transcription, enabling the monitoring of RNA synthesis and processing within live cells. Serving as an analog of uridine, BrUTP facilitates the measurement of transcription by labeling ribonucleic acids (RNA). Its utilization has contributed to the examination of gene expression regulation, RNA splicing, and RNA transport. During transcription, BrUTP replaces uridine triphosphate (UTP) and becomes part of the RNA molecule. Subsequently, fluorescent microscopy or flow cytometry can be employed to detect the presence of BrUTP in the RNA structure.
BrUTP (CAS 161848-60-8) References
- Ultrastructural analysis of nucleolar transcription in cells microinjected with 5-bromo-UTP. | Cmarko, D., et al. 2000. Histochem Cell Biol. 113: 181-7. PMID: 10817672
- Visualization of nascent transcripts on Drosophila polytene chromosomes using BrUTP incorporation. | Chang, WY., et al. 2000. Biotechniques. 29: 934-6. PMID: 11084849
- Electric field-mediated BrUTP uptake by mouse oocytes, eggs, and embryos. | Waksmundzka, M. and Debey, P. 2001. Mol Reprod Dev. 58: 173-9. PMID: 11139229
- Three-dimensional organization of active rRNA genes within the nucleolus. | Cheutin, T., et al. 2002. J Cell Sci. 115: 3297-307. PMID: 12140261
- [Localization with BrUTP labeling technique of RNA polymerase II transcription in meristematic cells of Allium cepa]. | Hu, B. and Xing, M. 1999. Shi Yan Sheng Wu Xue Bao. 32: 185-95. PMID: 12548785
- Nuclear structures in Tribolium castaneum oocytes. | Bogolyubov, DS., et al. 2013. Cell Biol Int. 37: 1061-79. PMID: 23686847
- Bromouridine triphosphate inhibits transcription termination and mRNA release by vaccinia virions. | Shuman, S. and Moss, B. 1989. J Biol Chem. 264: 21356-60. PMID: 2592381
- Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach. | Dhoondia, Z., et al. 2017. J Vis Exp.. PMID: 28362387
- Discontinuous transcription of ribosomal DNA in human cells. | Smirnov, E., et al. 2020. PLoS One. 15: e0223030. PMID: 32119673
- The localization of sites containing nascent RNA and splicing factors. | Pombo, A. and Cook, PR. 1996. Exp Cell Res. 229: 201-3. PMID: 8986598
- Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo. | Aoki, F., et al. 1997. Dev Biol. 181: 296-307. PMID: 9013938
- Labeling of RNA transcripts of eukaryotic cells in culture with BrUTP using a liposome transfection reagent (DOTAP). | Haukenes, G., et al. 1997. Biotechniques. 22: 308-12. PMID: 9043703
- Sites of rDNA transcription are widely dispersed through the nucleolus in Pisum sativum and can comprise single genes. | Thompson, WF., et al. 1997. Plant J. 12: 571-81. PMID: 9351243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BrUTP, 100 µl | sc-495784 | 100 µl | $260.00 |