Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Brm Double Nickase Plasmid (m): sc-426415-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Brm Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Brm Double Nickase Plasmid (m) and Brm Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Smarca2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Brm Antibody (E-1): sc-17828
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Brm Double Nickase Plasmid (m) | sc-426415-NIC | 20 µg | $410.00 | |||
Brm Double Nickase Plasmid (m2) | sc-426415-NIC-2 | 20 µg | $410.00 |
Mouse Smarca2 encodes Brm (SMARCA2), an ATP-dependent helicase subunit of the SWI/SNF (BAF) chromatin remodeling complex that repositions nucleosomes to regulate transcriptional programs. Brm helps coordinate enhancer and promoter accessibility for pathways controlling cell-cycle progression, lineage specification, DNA damage responses, and differentiation. Altered SWI/SNF activity, including disruption of SMARCA2/BRM function or complex composition, is linked to epigenetic dysregulation observed across multiple disease-relevant contexts such as cancer biology and neurodevelopmental processes. Smarca2 is therefore widely studied for its role in chromatin architecture, transcription factor cooperativity, and genome stability in mammalian cells.
Brm Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Smarca2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Smarca2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Smarca2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Smarca2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.