BPDE Antibody (8E11): sc-52624

BPDE Antibody (8E11) is a mouse monoclonal IgG1 kappa light chain antibody that detects the BPDE protein of various species by enzyme-linked immunosorbent assay (ELISA). anti-BPDE antibody (8E11) is available as a non-conjugated isotype antibody. Benzopyrene-7,8-diol-9,10-epoxide (BPDE) is a potent five-ring polycyclic aromatic hydrocarbon known for its mutagenic and highly carcinogenic properties, primarily arising from incomplete combustion processes such as those found in coal tar, automobile exhaust, tobacco smoke, and charbroiled foods. The significance of BPDE lies not only in its role as a carcinogen but also in its mechanism of action; BPDE is first activated by cytochrome P4501A1 to form (+)-benzo[a]pyrene 7,8-oxide, which is subsequently metabolized by epoxide hydrolase to yield (−)-benzo[a]pyrene-7,8-dihydrodiol. This compound ultimately forms the active carcinogen by further reacting with cytochrome P4501A1, resulting in benzopyrene diol epoxide. The electrophilic nature of the two carbons in the epoxide allows BPDE to intercalate and distort DNA, leading to covalent bonding with nucleophilic guanine nucleobases at the N2 position. This interaction is critical as BPDE results in increased micronuclei formation and apoptosis in cells, contributing to the development of various cancers, particularly lung cancer. BPDE monoclonal antibody (8E11) detection capabilities are essential for research into the mechanisms of carcinogenesis and the effects of environmental pollutants on human health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BPDE Antibody (8E11) References:

1. Immunohistochemical analysis of polycyclic aromatic hydrocarbon-DNA adducts in breast tumor tissue.  |  Santella, RM., et al. 2000. Cancer Lett. 154: 143-9. PMID: 10806302
2. Benzo(a)pyrene diolepoxide (BPDE)-DNA adduct levels in leukocytes of smokers in relation to polymorphism of CYP1A1, GSTM1, GSTP1, GSTT1, and mEH.  |  Lodovici, M., et al. 2004. Cancer Epidemiol Biomarkers Prev. 13: 1342-8. PMID: 15298956
3. XP-A cells complemented with Arg228Gln and Val234Leu polymorphic XPA alleles repair BPDE-induced DNA damage better than cells complemented with the wild type allele.  |  Porter, PC., et al. 2005. DNA Repair (Amst). 4: 341-9. PMID: 15661657
4. Protection by quercetin and quercetin-rich fruit juice against induction of oxidative DNA damage and formation of BPDE-DNA adducts in human lymphocytes.  |  Wilms, LC., et al. 2005. Mutat Res. 582: 155-62. PMID: 15781220
5. DNA polymerase kappa is specifically required for recovery from the benzo[a]pyrene-dihydrodiol epoxide (BPDE)-induced S-phase checkpoint.  |  Bi, X., et al. 2005. J Biol Chem. 280: 22343-55. PMID: 15817457
6. Membrane assembly of lactose permease of Escherichia coli.  |  Yamato, I. 1992. J Biochem. 111: 444-50. PMID: 1618733
7. Age-related changes in oxidative DNA damage and benzo(a)pyrene diolepoxide-I (BPDE-I)-DNA adduct levels in human stomach.  |  Lee, BM., et al. 2005. J Toxicol Environ Health A. 68: 1599-610. PMID: 16195216
8. The interaction effects of zinc and polygenic risk score with benzo[a]pyrene exposure on lung cancer risk: A prospective case-cohort study among Chinese populations.  |  Fu, M., et al. 2024. Environ Res. 250: 118539. PMID: 38401684
9. The dual effects of Benzo(a)pyrene/Benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide on DNA Methylation.  |  Zhao, C., et al. 2024. Sci Total Environ. 950: 175042. PMID: 39084379
10. BaP/BPDE suppresses homologous recombination repair in human trophoblast cells to induce miscarriage: The roles of lnc-HZ08.  |  Chen, W., et al. 2024. Environ Int. 191: 108975. PMID: 39190976
11. Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide.  |  Santella, RM., et al. 1984. Carcinogenesis. 5: 373-7. PMID: 6423306
12. A sensitive color ELISA for detecting polycyclic aromatic hydrocarbon-DNA adducts in human tissues.  |  Mumford, JL., et al. 1996. Mutat Res. 359: 171-7. PMID: 8618549