Bok CRISPR/Cas9 KO Plasmid (h): sc-407699
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Bok CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Bok genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bok CRISPR/Cas9 KO Plasmid (h) | sc-407699 | 20 µg | $397.00 |
Overview
BOK (BCL2-related ovarian killer) encodes Bok, a pro-apoptotic member of the BCL-2 protein family that contributes to intrinsic apoptosis by influencing mitochondrial outer membrane permeabilization and downstream caspase activation. Bok has been linked to endoplasmic reticulum and mitochondria-associated membrane biology, intersecting with stress-response pathways that integrate proteostasis and cell fate decisions. Through these roles, BOK is studied in contexts where apoptosis thresholds and cellular stress sensitivity are altered, including cancer biology, neurodegeneration-associated stress signaling, and tissue homeostasis. Its regulation and functional overlap with other BCL-2 family proteins make it a useful node for dissecting pathway redundancy and apoptotic priming.
Bok CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BOK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BOK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BOK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Bok protein expression.
This CRISPR knockout system enables efficient generation of BOK-deficient cell models for investigation of Bok signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BOK exon(s) critical for Bok function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BOK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Bok CRISPR/Cas9 KO Plasmid (h) and Bok CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BOK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Bok HDR Plasmid (h) and Bok HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BOK homology arms to support homology-directed repair at defined BOK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.