Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMP2K CRISPR/Cas9 KO Plasmid (h): sc-405196
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP2K CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMP2K genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMP2K Antibody (E-8): sc-514681
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP2K CRISPR/Cas9 KO Plasmid (h) | sc-405196 | 20 µg | $397.00 |
Overview
BMP2K (BMP-2 inducible kinase) encodes a serine/threonine kinase implicated in intracellular trafficking and membrane dynamics, with reported roles in clathrin-mediated endocytosis and regulation of adaptor protein complexes. Its activity has been linked to modulation of erythroid maturation and cellular differentiation programs, consistent with BMP-responsive signaling contexts. BMP2K-associated phosphorylation events can influence receptor internalization and downstream signaling amplitude, connecting it to broader networks controlling proliferation and stress responses. Dysregulated endocytic and differentiation pathways involving BMP2K have been explored in studies of hematologic phenotypes and cancer-associated signaling rewiring.
BMP2K CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BMP2K gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BMP2K together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BMP2K open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMP2K protein expression.
This CRISPR knockout system enables efficient generation of BMP2K-deficient cell models for investigation of BMP2K signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BMP2K exon(s) critical for BMP2K function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BMP2K genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMP2K CRISPR/Cas9 KO Plasmid (h) and BMP2K CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BMP2K locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMP2K HDR Plasmid (h) and BMP2K HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BMP2K homology arms to support homology-directed repair at defined BMP2K target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.