Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Blr1 Double Nickase Plasmid (h): sc-402672-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Blr1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Blr1 Double Nickase Plasmid (h) and Blr1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CXCR5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Blr1 Antibody (C-3): sc-373775
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Blr1 Double Nickase Plasmid (h) | sc-402672-NIC | 20 µg | $410.00 | |||
Blr1 Double Nickase Plasmid (h2) | sc-402672-NIC-2 | 20 µg | $410.00 |
CXCR5 encodes the chemokine receptor Blr1 (CD185), a seven-transmembrane GPCR that binds CXCL13 to guide B cells and T follicular helper cells into B-cell follicles and germinal centers. Ligand engagement activates heterotrimeric G-protein signaling with downstream PI3K–AKT, MAPK, and calcium flux pathways that coordinate chemotaxis, adhesion dynamics, and lymphoid tissue organization. CXCR5-dependent trafficking and positioning are central to humoral immunity, affinity maturation, and lymphoid neogenesis, and altered CXCR5/BLR1 signaling is studied in immune dysregulation and B-cell lineage malignancies. In addition, CXCR5 expression on circulating T cells is widely used to interrogate follicular helper–like phenotypes and their roles in inflammation and autoimmunity.
Blr1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CXCR5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CXCR5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CXCR5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CXCR5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.