Blood Group Lewis b Antibody (2-25LE): sc-51513

Blood Group Lewis b Antibody (2-25LE) is a mouse monoclonal IgG1 antibody that detects the Lewis b blood group antigen of human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Lewis b antibody (2-25LE) is available in a non-conjugated form, providing flexibility for various experimental setups. The Lewis b antigen plays a crucial role in cell-cell interactions and is essential for proper immune response and cellular communication. Lewis b antigen is primarily located on the surface of red blood cells and other cell types, where Lewis b serves as a recognition site for selectins, which are important for leukocyte trafficking and adhesion during immune responses. The presence of Lewis b antigen on cell surfaces is vital for the regulation of these processes, as Lewis b influences how cells interact with one another and with the extracellular matrix. Additionally, Lewis b antigen is involved in the modulation of inflammatory responses, making Lewis b a significant target for studies related to immunology and hematology. Understanding the function and distribution of Lewis b antigen can provide insights into various pathological conditions, including blood group incompatibilities and certain diseases where cell adhesion plays a pivotal role.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Blood Group Lewis b Antibody (2-25LE) References:

1. Alteration of sialyl Lewis epitope expression in pterygium.  |  Creuzot-Garcher, C., et al. 1999. Invest Ophthalmol Vis Sci. 40: 1631-6. PMID: 10393028
2. Expression and transcriptional regulation of the human alpha1, 3-fucosyltransferase 4 (FUT4) gene in myeloid and colon adenocarcinoma cell lines.  |  Taniguchi, A., et al. 2000. Biochem Biophys Res Commun. 273: 370-6. PMID: 10873613
3. P-selectin glycoprotein ligand-1 and E-selectin ligand-1 are differentially modified by fucosyltransferases Fuc-TIV and Fuc-TVII in mouse neutrophils.  |  Huang, MC., et al. 2000. J Biol Chem. 275: 31353-60. PMID: 10882744
4. Human alpha (1,3)-fucosyltransferase IV (FUTIV) gene expression is regulated by elk-1 in the U937 cell line.  |  Withers, DA. and Hakomori, SI. 2000. J Biol Chem. 275: 40588-93. PMID: 11006292
5. Potent induction of alpha(1,3)-fucosyltransferase VII in activated CD4+ T cells by TGF-beta 1 through a p38 mitogen-activated protein kinase-dependent pathway.  |  Wagers, AJ. and Kansas, GS. 2000. J Immunol. 165: 5011-6. PMID: 11046029
6. Lewis antigen alterations in gastric cancer precursors.  |  Torrado, J., et al. 1992. Gastroenterology. 102: 424-30. PMID: 1732113
7. Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization.  |  Richman, PI. and Bodmer, WF. 1987. Int J Cancer. 39: 317-28. PMID: 2434440
8. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens.  |  Bara, J., et al. 1988. Biochem J. 254: 185-93. PMID: 2460087

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Blood Group Lewis b Antibody (2-25LE) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Blood Group Lewis b Antibody (2-25LE) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Blood Group Lewis b Antibody (2-25LE) Specifications

Alternate Product Name: Blood Group Lewis b

Product Clone Name: 2-25LE

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Mucin

Epitope Species: human

Product Reactivity/Detection: Lewis b blood group antigen

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Blood Group Lewis b Antibody (2-25LE): N/A